Also known as the Cenacle, the Last Supper Room is located in Jerusalem, south of the Zion Gate in the walls of the Old City on the upper floor of a two-storey building.

According to Christian tradition, this room is held to be the site of the Last Supper – the meal that Jesus shared with his apostles the night before his crucifixion, and also the room in which the Holy Spirit alighted on the eleven apostles. It is also thought to be the place where the apostles stayed when they were in Jerusalem.